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. 2021 Feb 15;16:136. doi: 10.1186/s13018-021-02268-x

Fig. 5.

Fig. 5

Vangl2 silencing can eliminate the effect of Wnt5a on inflammatory activation. a RT-qPCR analysis shows Wnt5a-overexpression efficiency of Wnt5a plasmid in ATDC5. b Effects of Vangl2 and Wnt5a on the expression of Sox9 and Col-2 were measured by Western blotting. c Protein expression of MMP3, MMP9, and MMP13 were also measured by Western blotting. GAPDH was used as a loading control. ns, not statistically significant, *P < 0.05, **P < 0.01; nc/NC, negative control; +, overexpression; -, silencing