Table 2.
Metagenomic reads classified as chitinase-encoding gene sequences with MG-RAST
| Dataset | Sample category | # Reads MG-RASTa | # Blastb (%) |
|---|---|---|---|
| Octocoral metagenome | Eunicella gazella (healthy) | 94 | 68 (72.3%) |
| Eunicella gazella (necrotized) | 59 | 47 (79.7%) | |
| Eunicella verrucosa | 1458 | 795 (54.5%) | |
| Leptogorgia sarmentosa | 127 | 100 (79.4%) | |
| Sediments | 401 | 124 (30.9%) | |
| Seawater | 599 | 317 (52.9%) | |
| Sponge metagenome | Spongia officinalis | 48 | 12 (25.0%) |
| Sediments | 455 | 103 (22.6%) | |
| Seawater | 257 | 103 (40.1%) |
aNumber of unassembled metagenomic reads classified by MG-RAST as endo-chitinase (EC 3.1.2.14) coding sequences
bProportion of chitinase reads classified by MG-RAST which were assigned to the domain Bacteria and returned chitinase-specific closest hits after blastx searches on NCBI. Figure 4 shows the taxonomic affiliation of these reads in detail