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. 2021 Jan 14;12(2):236–241. doi: 10.1021/acsmedchemlett.0c00605

Figure 3.

Figure 3

Involvement of the ubiqutin-proteasome system in the PROTAC(H-PGDS)-1-induced degradation of H-PGDS protein. (a) KU812 cells were incubated with 1 μM PROTAC(H-PGDS)-1 or the ligand mixture (TFC-007 and pomalidomide, 1 μM each) for 6 h. (b) Competition assay using an excess amount of pomalidomide with PROTAC(H-PGDS)-1 in KU812 cells. Cells were incubated with 100 nM PROTAC(H-PGDS)-1 and/or 10 μM pomalidomide for 6 h. (c) Effect of MG132 and MLN7243 on the protein knockdown activity of PROTAC(H-PGDS)-1 in KU812 cells. Cells were incubated with 100 nM PROTAC(H-PGDS)-1 in the presence or absence of 10 μM MG132 or 10 μM MLN7243 for 6 h. The H-PGDS/β-actin ratio was normalized by the vehicle control as 100. The data in the bar graphs are means ± SD (n = 3). *P < 0.01 compared to vehicle-treated control in a two-tailed Student’s t test.