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. 2021 Feb 9;6(1):e01125-20. doi: 10.1128/mSystems.01125-20

TABLE 2.

Endophytic bacterial load in root and shoot tissues of common bean (Phaseolus vulgaris) inoculated with CTX-M-15-producing Enterobacterales isolated from commercial vegetables

Inoculating straind CFU/g tissue ± SD
Root Shoot
K. pneumoniae ALF301a 1.6 ± 0.1 × 105 6.9 ± 0.4 × 102
K. pneumoniae RUC232a 8.7 ± 0.5 × 104 3.8 ± 0.3 × 102
E. cloacae ESP151b 6.4 ± 0.4 × 104 2.1 ± 0.1 × 103
E. coli REP215 3.9 ± 0.4 × 104 7.0 ± 0.9 × 101
E. coli ESP110 3.2 ± 0.6 × 104 3.1 ± 0.5 × 102
E. coli REP237 5.1 ± 0.5 × 103 7.5 ± 1.0 × 101
A. baumannii ATCC 19606 -c -c
a

Significantly higher bacterial counts in the root (P < 0.05).

b

Significantly higher bacterial counts in the shoot (P < 0.05).

c

Undetectable bacterial colonies.

d

Common beans (Phaseolus vulgaris) grains were surface-sterilized and, after a two-day germination, sprouts were incubated for 30 min with bacterial suspension (OD600 = 1.5) and transferred to plant culture bottles with Murashige and Skoog medium. Endophytic bacterial load in root and shoot tissue was assessed at 15 days after inoculation. All assays were performed in triplicate. Acinetobacter baumannii ATCC 19606 was used as a negative control for endophytic colonization.