TABLE 2.
Recovery of 14C label in community substrate incubations after 3 days
| Compounda | Dosageb (mg C · liter−1) | % Radioactivity recovered inc |
||
|---|---|---|---|---|
| Filtered biomassd | Trapped gas phasee | Filtratef | ||
| Phenol (set 1) | 0.1 | 8.4 ± 4.9 | 35.6 ± 5.3 | 53.3 ± 2.6 |
| 1.0 | 8.5 ± 0.6 | 36.2 ± 2.6 | 51.7 ± 8.0 | |
| 10 | 15.8 ± 4.9 | 44.5 ± 6.5 | 33.1 ± 6.9 | |
| Abiotic control | 1.3 ± 0.1 | 3.5 ± 0.0 | 90.2 ± 0.2 | |
| Phenol (set 2) | 0.1 | 12.4 ± 1.0 | 29.6 ± 1.0 | 53.9 ± 9.0 |
| 1.0 | 11.5 ± 1.5 | 29.7 ± 1.5 | 54.0 ± 6.9 | |
| 10 | 11.9 ± 4.0 | 43.4 ± 6.6 | 40.3 ± 1.3 | |
| Abiotic control | 1.9 ± 0.1 | 1.8 ± 0.8 | 97.7 ± 2.6 | |
| 1-Octanol | 0.1 | 6.5 ± 2.0 | 28.2 ± 9.8 | 65.3 ± 6.1 |
| 1 | 6.7 ± 0.3 | 35.7 ± 25.4 | 57.6 ± 8.9 | |
| 10 | 6.7 ± 0.3 | 39.4 ± 15.9 | 53.9 ± 5.5 | |
| Abiotic control | 2.5 ± 0.1 | 14.8 ± 4.8 | 74.9 ± 13.3 | |
a
14C-uniformly labeled phenol and 14C-C1-labeled 1-octanol, in addition to nonlabeled versions of the same compound.
b
Dosage of nonlabeled compound, in addition to 14C-labeled compound. For phenol, 5,000 dpm · ml−1 (∼4 ng · ml−1); for 1-octanol, 1,300 dpm · ml−1.
c
Mean values from triplicate incubations ± one standard deviation. Values corrected for total 14C recovery.
d
C in biomass (%): counts of filter after passing solution.
e
C in CO2 (%): counts of NaOH solution through which gas phase of incubation was passed.
f
Substrate (%): counts of solution after passing through filter.