Fig. 7.

PEG-CeNP treatment protected against A1 astrocyte alteration after ICH. Representative RT-qPCR analysis of A1 astrocyte markers expression. a C3 mRNA expression. b Amigo2 mRNA expression. c Serping1 mRNA expression. n = 6/group, * p < 0.01 versus Sham. ^ p < 0.01 versus ICH 3d. # p < 0.01 versus ICH 7d. d C3 mRNA expression in PEG-CeNP-treated mice at 7 days post ICH. n = 6/group, * p < 0.01 versus Sham, # p < 0.01 versus ICH + vehicle. e Representative images of GFAP and C3 double immunostaining in cultured astrocytes (treated with H₂O₂). Lens: × 400; Scale bar: 50 μm. f Representative images of GFAP and C3 double immunostaining in brain sections at 7 days post ICH. Lens: × 100, × 200; Scale bar: 200 μm (yellow), 100 μm (white). g C3 mRNA expression in cultured astrocytes. n = 6/group, * p < 0.05 versus Control, # p < 0.05 versus H₂O₂ + vehicle. h Representative Western blot band showed the expression of nuclear NF-κB p65 in cultured astrocytes, n = 6/group, * p < 0.05 versus Control, # p < 0.05 versus H₂O₂ + vehicle. i Representative images of NF-κB p65 and GFAP double immunostaining in cultured astrocytes (treated with H₂O₂). Lens: × 400; Scale bar: 25 μm