FIGURE 1.

Reverse-phase HPLC chromatogram of the venom of O. supermirabilis. The soluble venom of O. supermirabilis (obtained from 2 mg crude soluble venom) was fractionated using a reverse-phase analytical C₁₈ column (5C₁₈MS, 4.6 × 250 mm) equilibrated in 0.1% TFA, and eluted with a linear gradient of acetonitrile (solution B) starting after 5 min from 0 to 60% in 0.1% TFA, run for 60 min at a flow rate of 1 ml/min. The fraction peak #59 in the figure was the fraction that gave positive results in modifying currents in hK_v1.5, and was further purified to homogeneity (Inside figure) starting after 5 min from 20 to 60% CH₃CN during 40 min at a flow rate of 1 ml/min.