TABLE 1.

Amino acid sequencing and molecular masses of endoproteolytic fractions from Osu1.

^aMolecular mass after subtraction of –57 Da (Cysteine carbamidomethylation by iodoacetamine) to the molecular masses of the alkylated fractions containing Cys (HPLC separation and molecular masses of alkylated fractions are shown in Supplementary Figure S1).

^bA subtraction of an extra –8 Da to the alkylated Osu1 was performed assuming four disulfide bridges.

^cNon-alkylated peptide fragment. The experimental molecular masses in parenthesis are shown in Supplementary Figure S1.