TABLE1.

List of Primers Used for Amplification and Sequencing of HBV Genome

Primer Name	Primer Sequences	Location (nt)*
HBVP1†,‡ (Sense)	5′-CCGGAAAGCTTGAGCTCTTCTTTTTCACCTCTGCCTAATCA -3′	1821-1841
HBVP2†,‡ (Antisense)	5′-CCGGAAAGCTTGAGCTCTTCAAAAAGTTGCATGGTGCTGG -3′	1806-1825
F10§,║ (Sense)	5′ -GACCACCAAATGCCCCTATC -3′	2297-2317
F3║ (Sense)	5′-CGCCTCATTTTGTGGGTCAC-3′	2801-2820
F4║ (Sense)	5′-CTCAGGCCATGCAGTGGAA- 3′	3164-3182
CF║ (Sense)	5′-ACTGTTCAAGCCTCCAAGCT-3′	1861-1880
R4║,¶ (Antisense)	5′-AGAGGACAAACGGGCAACA-3′	462-480
R2║ (Antisense)	5′-AAATTACCACCCACCCAGG-3′	2109-2127
R3║ (Antisense)	5′-AACTGGAGCCACCAGCAG- 3′	57-74
R5║ (Antisense)	5′-AAAGCCCAAAAGACCCACAAT-3′	997-1017
F5║,¶ (Sense)	5′-GATGTGTCTGCGGCGTTTTA- 3′	376-395
R9§,║ (Antisense)	5′-TAGGAGTTCCGCAGTATGGA- 3′	1265-1284
R10║ (Antisense)	5′-CAGCCTCCTAGTACAAAGAC-3′	1761-1780
F7║ (Sense)	5′-TGTGCACTTCGCTTCACCTC-3′	1578-1597

^*nt positions are given according to HBV sequence with accession no. AF121242 obtained from GenBank.

^†Primers used for full-genome amplification of HBV isolates.

^‡Günther et al.

^§Primers used for amplification of the complete surface region (2848–835 nt).

^║Primers used for sequencing of full-length as well as complete surface region.

^¶Primers used for viral load determination by real-time PCR.