Figure 3.

PNU-282987 and GTS-21 inhibit Alternaria-mediated airway inflammation. C57/BL6J mice intranasally received an extract of Alternaria (AA), AA with or without PNU-282987/GTS-21 on days 1 to 4. (A) levels of IL-33 RNA in lung and protein in BALF were assessed by qPCR and ELISA respectively. (B) Lung tissues were stained by PAS staining. Scoring for histopathology and PAS was assessed by light microscopy (×200, Scale bars at 100 μm). (C) Levels of eosinophils and ILC2s in BALF, (D) CD4⁺ T cells and CD19⁺ B cells in lung were determined by flow cytometry. Data are representative of at least four independent experiments and presented as means ± s.e.m (n = 4 - 5; *P < 0.05; **P < 0.01; ***P < 0.001).