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. 2021 Feb 12;9:e10553. doi: 10.7717/peerj.10553

Table 1. The effects of incubation temperature and substrate moisture on incubation duration, hatching success, hatchling malformation rates and survival rates in Mauremys reevesii.

Incubation temperature (°C) Substrate moisture No. of fertilized eggs Hatching success (%) Malformation rate of hatchlings (%) Survival rate of hatchlings (%) Incubation duration (range)
23 1:0.5 19 73.7 (14/19) 21.4 (3/14) 35.7 (5/14) 117.1 ± 2.4 (115.0∼121.0)
1:0.9 22 81.8 (18/22) 22.2 (4/18) 66.7 (12/18) 113.7 ± 3.9 (107.0∼119.0)
1:1.2 18 72.2 (13/18) 23.1 (3/13) 61.5 (8/13) 113.7± 2.9 (109.0∼117.0)
26 1:0.5 19 100.0 (19/19) 10.5 (2/19) 94.7 (18/19) 86.3 ± 3.2 (82.0∼92.0)
1:0.9 16 87.5 (14/16) 28.6 (4/14) 92.9 (13/14) 83.9 ± 2.5 (79.0∼88.0)
1:1.2 23 95.7 (22/23) 13.6 (3/22) 90.9 (20/22) 84.8 ± 2.5 (80.0∼88.0)
29 1:0.5 20 80.0 (16/20) 12.5 (2/16) 100.0 (16/16) 72.1 ± 1.8 (70.0∼75.0)
1:0.9 27 85.2 (23/27) 8.7 (2/23) 100.0 (23/23) 71.7 ± 2.4 (68.0∼75.0)
1:1.2 30 96.7 (29/30) 3.4 (1/29) 89.7 (26/29) 68.9 ± 3.3 (63.0∼73.0)
32 1:0.5 27 48.1 (13/27) 15.4 (2/13) 92.3 (12/13) 62.2 ± 2.4 (59.0∼66.0)
1:0.9 26 53.8 (14/26) 0 (0/14) 85.7 (12/14) 59.5 ± 2.4 (56.0∼63.0)
1:1.2 23 34.8 (8/23) 12.5 (1/8) 87.5 (7/8) 60.0 ± 2.3 (56.0∼63.0)
35 1:0.5 23 0 (0/23)
1:0.9 24 4.2 (1/24) 100.0 (1/1) 0 (0/1)
1:1.2 20 15.0 (3/20) 100.0 (3/3) 0 (0/3)

Note:

“−” indicates no data. Survival rates of hatchlings were calculated at 12 months of age.