Table 1. Chemical characteristics of alfalfa (g/100 g).

Diets type	Protein	Crude fiber	Crude fat	Crude ash
HV (alfalfa)	19.62 ± 0.23	26.75 ± 0.24	4.86 ± 0.11	8.34 ± 0.02
NHV (pellet feed)	27.22 ± 0.09	4.97 ± 0.03	2.17 ± 0.05	9.03 ± 0.06

Note:

HV (alfalfa): the herbivorous snails reared with alflafa; NHV(pellet feed): the non-herbivorous snails reared with pellet feed. The data on the composition of the pellet feed come from the manufacturer (Takara sakana-ii). The composition of the alfalfa was determined in laboratory as follwing methods: (1) The NDF and ADF levels were measured as described by Van Soest, Robertson & Lewis (1991). (2) The crude protein (CP) content was measured by the Kjeldahl N method (AOAC 2000). (3) Soxhlet immersion extraction was used for measure crude fat. (4) Crude ash was measured by firing the sample for 30 min under 550 °C in muffle furnace.