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PROBIT REGRESSION AND LOD AMPLIFICATION CURVES. A. Probit regression curves of the “pure LOD”. The dashed lines represent the upper and lower 95 % confidence intervals. B. “Pure LOD” for in-vitro transcribed RNA. C. “Extracted LOD” for in-vitro transcribed RNA, 500 copies/ml. D. “Extracted LOD” for the SARS-CoV-2 standard (Exact Diagnostics), 500 copies/ml. E. “Extracted LOD” for whole virus derived from a patient specimen, 375 copies/ml.
