Table 2.
S and E gene primers and probe sequences for one step RT-qPCR with sequence identity to SARS-CoV-2, SARS-CoV and MERS.
| Sequence identity (nt) |
||||||
|---|---|---|---|---|---|---|
| Gene | Primer | Sequence (5’>3’) | SARS-CoV-2 | SARS-CoV | MERS | Amplicon |
| S | Virion Sgene-F2 | AAC TCA ATT ACC CCC TGC ATA C | 22/22 | 14/22 | Deleted (0/23) | 167bp |
| Virion Sgene-R2 | TAG TAC CAT TGG TCC CAG AGA CA | 23/23 | 2/23, 18/23 deleted | 15/23 | ||
| SgeneProbe-2 | TCAGATCCTCAGTTTTACATTCAACTCAGGACTTG | 35/35 | 21/35 | 10/35 | ||
| E | EgeneF2 RQ-PCR | TCA TTC GTT TCG GAA GAG ACA G | 22/22 | 21/22 | 11/22 | 103bp |
| EgeneR2 RQ-PCR | GCG CAG TAA GGA TGG CTA GT | 20/20 | 20/20 | 12/20 | ||
| EgeneProbe-2 | AGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCT | 34/34 | 34/34 | 10/34 | ||