Table 3.
Performance characteristics of standard curves – S gene assay. Mean, standard deviation, 95 % CI and CV are shown for the Ct value of each standard (columns 2–9), slope and correlation co-efficient, R2 (columns 10–11) from 6 runs performed on different days. S gene (A), E gene (B).
| 108 | 107 | 106 | 105 | 104 | 103 | 102 | 101 | Slope | R2 | |
|---|---|---|---|---|---|---|---|---|---|---|
| Mean | 9.4 | 12.96 | 16.05 | 19.53 | 23.12 | 26.64 | 29.93 | 33.33 | −3.425 | 0.9995 |
| SD | 0.04 | 0.14 | 0.09 | 0.16 | 0.19 | 0.32 | 0.24 | 0.36 | 0.044 | 0.0006 |
| 95 % CI | 9.36, 9.45 | 12.81, 13.10 | 15.96, 16.14 | 19.36, 19.69 | 22.92, 23.32 | 26.31, 26.98 | 29.68, 30.18 | 32.95, 33.72 | −3.47, -3.38 | 0.998, 1.000 |
| CV | 0.45 % | 1.09 % | 0.54 % | 0.8 % | 0.83 % | 1.2 % | 0.8 % | 1.09 % | 1.28 % | 0.06 % |