Table 5.

Estimation of the “pure LOD” by probit analysis. Copies/reaction of in-vitro transcribed RNA, replicates analyzed and number detected are shown.

S in-vitro transcribed RNA			E in-vitro transcribed RNA
Copies/reaction	Replicates	Detected	Copies/reaction	Replicates	Detected
1	42	29	1	45	24
3	42	40	3	45	40
5	56	54	5	50	49
10	48	48	10	12	12
16	12	12	15	12	12
32	12	12	25	12	12
63	12	12