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. 2021 Jan 27;10:592884. doi: 10.3389/fonc.2020.592884

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Copyright © 2021 Ma, Huang, Huang, Li, Li, Huang, Zhong and Cao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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circ_0014717 sponged miR-668-3p in hepatocellular carcinoma (HCC) cells. (A, B) Top six miRNA candidates in HCC cell lysates were tested by real-time PCR. Multiple miRNAs were pulled down using circ_0014717 probe. (C) The expression level of miR-668-3p in HCC cells infected with LV-circ_0014717 or LV-NC. (D) The putative binding sites between circ_0014717 and miR-668-3p. (E, F) Luciferase activity was evaluated in HCC cells co-transfected with circ_0014717-WT or circ_0014717-MUT reporter and miR-668-3p inhibitors or its scramble control (NC). (G) The expression of miR-668-3p in HCC carcinoma and normal adjacent tissues. (H) The expression of miR-668-3p in HCC cells (Huh-7, Hep-3B, HepG2, SMMCC-7721, MHCC97L) and LO2 cells. *P < 0.05.