TABLE 1.

Plating guideline for Minibrain formation.

	No. of cells	Medium volume	Average number of spheres	Requiered volume of cell suspension
6-well	1*106	3 ml	95 ± 34 (N = 12 wells)	2*106/X
6-well	2.5*105	3 ml	49 ± 13 (N = 129 wells)	5*105/X
24 well	2*104	500 μl	1 or 2	4*105/X

X, number of living cells/ml in cell suspension.