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. Author manuscript; available in PMC: 2021 Feb 15.
Published in final edited form as: Mol Cancer Ther. 2020 May 5;19(7):1539–1549. doi: 10.1158/1535-7163.MCT-19-0776

Fig. 3: DCLK1-isoform2 overexpressing AsPC1 cells secrete cytokines/chemokines to polarize macrophages in a dual culture system.

Fig. 3:

A) Dual cell culture model. B) Protein expression of CD163 and CD206 (M2 markers) in the total protein lysates of M1-macrophages dual cultured with AsPC1 vector control cells or M1-macrophages dual cultured with AsPC1-DCLK1-isoform2 cells. C) Human cytokine array by dot blot analysis, conditioned media collected from AsPC1 vector control cell culture or AsPC1-DCLK1-isoform2 overexpressing cell culture utilized for the array. Cytokines/chemokines with 2-fold differences in their increase were numbered in the blot and named. D) Arrays were quantified using the densitometric analysis to represent a fold change in a bar graph.