Dipolar couplings extracted from pseudo-4D N–Cx
and H–CO
TREDOR with different sets of mixing times. (A) All contacts found
in SH3, with dipole coupling measured by fitting subsets of one mixing
time (black) and two mixing times (blue) plotted against the fit with
the complete set. Site-specific TREDOR fitting for (B,D) Val9 N (C,E)
Trp42Nϵ, with distances from the crystal structure
as an inset. Bessel function fits are shown with three mixing times
(solid line), sets of two mixing times (dashed lines), and single
mixing time (dotted lines). (F) Exemplary TREDOR H–CO strips
for Trp42Nϵ, Val9 N, and Gly51 N with a mixing time
of 1.2 ms.