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. 2021 Jan 26;20(2):e13299. doi: 10.1111/acel.13299

FIGURE 4.

FIGURE 4

DON prolongs graft survival in old recipients by targeting 7CD4+ T‐cell metabolism. (a) Skin allografts from young DBA mice were transplanted onto young and old C57BL/6 mice. Recipients were treated with 1.6 mg/kg DON g.o.d. or PBS starting on the day of transplantation. Graft survival was monitored daily over 60 days. (b) CD8+ T cells of recipient mice were depleted with anti‐CD8 monoclonal antibody. Graft survival was recorded and compared to wild‐type C57BL/6 mice. (c) CD4+ T cells isolated from young or old C57BL/6 mice were adoptively transferred to old recipients and graft survival monitored after DON or PBS treatment. (d) Fully mismatched young skin transplant recipients were treated with triple treatment (DON, 2‐DG and metformin), or PBS. An additional group of old recipients was treated with DON only. Statistical significance for survival data was determined by log‐rank Mantel–Cox test, n = 7/group. Asterisks indicate p‐values *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001, only significant values are shown