Skip to main content
. 2021 Jan 17;20(2):e13307. doi: 10.1111/acel.13307

FIGURE 2.

FIGURE 2

Lycorine hydrochloride accelerates the clearance of γH2AX foci and maintains genome integrity. (a,b) Lycorine hydrochloride accelerates the clearance of γH2AX foci. HCA2 cells were treated with 2 Gy of X‐ray radiation, incubated with lycorine hydrochloride at a concentration of 1 μM, and then immunostained for γH2AX foci at the indicated time points. Representative images are shown in (a), and the quantification of cells with >10 γH2AX foci is shown in (b). (c) Lycorine hydrochloride promotes the genomic stability of HCA2 cells. Representative images of the analysis of genomic stability of the HCA2 cells treated with 1 μM lycorine hydrochloride for 24 h (left panel) based on a comet assay. The tail moment of at least 50 cells for each group was quantified using Comet Score software (Sumerduck, VA, USA) (right panel). Error bars in (b) represent the SEM. Error bars in (c) represent the SEM. p < 0.001, t test. All experiments were repeated at least three times. LH, lycorine hydrochloride.