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. 2021 Jan 17;20(2):e13307. doi: 10.1111/acel.13307

FIGURE 5.

FIGURE 5

SIRT1 depletion abrogates the lycorine hydrochloride‐mediated suppression of the onset of SIPS and the expression of SASP factors. (a,b) Knocking down SIRT1 abrogated the lycorine hydrochloride‐mediated suppression of the onset of SIPS. The procedure for the induction of SIPS is described in Figure 1. SIRT1‐depleted cells were treated with lycorine hydrochloride one day before X‐ray irradiation. SA‐β‐gal staining was performed on day 14 post‐IR, followed by the quantification of SA‐β‐gal‐positive cells. (c) Lycorine hydrochloride‐induced suppression of SASP factor expression was partially abrogated by SIRT1 depletion. SIRT1‐depleted cells were treated with lycorine hydrochloride (1 μM) for one day before X‐ray irradiation. The cells were then collected on day 6 post‐IR. The relative mRNA expression of the indicated SASP factors was analyzed by quantitative RT‐PCR. Error bars represent SD **p < 0.01, ****p < 0.001, t test. All experiments were repeated at least three times. LH, lycorine hydrochloride.