FIGURE 2.

prdh‐1 activity is required for sperm‐specific fertility defects in alh‐6 mutants. (a–f) prdh‐1 mutation rescues reduced male sperm size (a), impaired male sperm activation (b), reduced brood size in hermaphrodite (c), increased number of unfertilized oocytes in hermaphrodite (d), reduced sperm number in hermaphrodite (e), and male sperm competition (f) in alh‐6 animals. Statistical comparisons of sperm size, progeny, unfertilized oocytes, and sperm number were done by ANOVA. Comparisons of sperm activation and competition assays were done using Fisher's exact test with adjusted p‐value cutoffs. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. All studies performed in minimum of three biological triplicates; refer to Table S1 for n for each comparison