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. 2021 Mar;110(3):1042–1053. doi: 10.1016/j.xphs.2020.11.039

Fig. 2.

Fig. 2

Characterization of five NRRV P[8] antigens evaluated in the vaccine formulation developability assessments. SDS-PAGE analysis of purity and composition of the NRRV P[8] parent antigen and variants under (a) non-reducing and (b) reducing conditions. Immunochemical binding analysis of P[8] antibody binding to NRRV P[8] parent antigen and variants as measured by BLI including (c) Representative sensorgram for Pp P[8]-mAb interaction, and (d) Binding and kinetic parameters measured using Octet Red96 instrument (values shown are n = 3, 1 SD). For gels, lanes include (MW) Molecular Weight marker in kDa, (1) E. coli P[8], (2) Pp P[8] (truncated), (3) Pp P[8], (4) Pp P[8]-N85A,N151A, and (5) Pp P[8]-N85Q,N151Q,C171S. Solid dividing line: images of two different gels; and dashed dividing line: images of different parts of same gel. The presence of N-linked glycosylated species was confirmed by Endo H enzyme treatment as shown in Supplementary Fig. S1.