Fig. 3.
Effect of the vaccine preservative thimerosal on the integrity of the primary structure and conformational stability of NRRV P[8] parent protein and variants as measured by (a) intact protein mass analysis, (b) DSC, and (c) extrinsic fluorescence spectroscopy vs. temperature. For determination of the mean Tm values, n = 3 samples were measured with observed 1 SD values of ± −0.1 °C for DSC and ±0.1–0.7 °C for extrinsic fluorescence spectroscopy. Samples were prepared in 10 mM PBS buffer, pH 7.2. See Supplemental Table S1 for summary of Tm ±1 SD values.