Table 4.

Synergistic action of F2 on fat reduction in 3T3-L1cells.

F2 and ingredients	Amount of ingredients in 60 μg/mL of F2	Lipid inhibition (%)
Control	—	0.00 ± 3.08
GT	7.481 μg/ml	5.41 ± 6.59
RV	22.44 μg/ml	14.01 ± 2.36
OJ	29.93 μg/ml	−7.57 ± 5.96
RJ	0.074 μg/ml	−4.27 ± 4.58
Lemon	0.074 μg/ml	−1.83 ± 3.93
F2	60 μg/ml	26.31 ± 4.73
Cumulative inhibition of lipid production by the ingredients of F2 = 5.75%

The synergistic effect of F2 by its ingredients on lipid production by 3T3-L1 adipocytes was determined by ORO assay. The lipid accumulations (expressed as the percentage of control) are presented as means ± SD of triplicate experiments. GT: Geranium thunbergii; RV: Rhus verniciflua; OJ: Orostachys japonicus; RJ: Royal jelly.