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Mapping the full-length sequence of lncRNA Lx8-SINE B2. (a) Schematic of the 3′-rapid amplification of cDNA ends (RACE) (left) and 3′ RACE result for lncRNA Lx8-SINE B2 (right). (b) Schematic of the 5′ RACE and its result for lncRNA Lx8-SINE B2. (c) DNA sequencing of RACE using a universal primer in pEASY-T1 vector. (d) Validation of lncRNA Lx8-SINE B2 transcript size by PCR from cDNA. M, DNA marker.
