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. 2021 Feb 6;2021:6657597. doi: 10.1155/2021/6657597

Figure 2.

Figure 2

Genomic location and expression of lncRNA Lx8-SINE B2. (a) Schematic of the mouse lncRNA Lx8-SINE B2 locus on chromosome 13. lncRNA Lx8-SINE B2 is between Adgrv1 and Lysmd3. There are three exons and some retrotransposon fragments of LINE or SINE in lncRNA Lx8-SINE B2. RT–qPCR primers were indicated below. (b) The expression level of lncRNA Lx8-SINE B2, Oct4 Sox2, and Esrrb in mESCs in the presence or absence of LIF for 2-4 days, as measured by RT–qPCR and normalized to Gapdh levels. Biological-triplicate data (n = 3 dishes) are presented as mean ± s.e.m. (c) qPCR analysis of the expression level of lncRNA Lx8-SINE B2 in mouse ESCs and MEF cells. ∗∗∗p < 0.001 according to two-sided Student's t-test (d) Expression analysis of lncRNA Lx8-SINE B2 in ESCs cultured under serum/LIF, 2i/LIF or 2i condition. Biological-triplicate data (n = 3 extracts) are presented as mean ± s.e.m.