Figure 5.

ALX significantly promoted intracellular ROS overproduction in pancreatic β-cells. (a) Intracellular ROS levels were detected by flow cytometry after exposure to vehicle and ALX (1.5, 3, and 6 mM) for 24 h. (b) Intracellular ROS generation was detected by fluorescence microscopy (scale bar: 50 μm) after treatment with vehicle and ALX (1.5, 3, and 6 mM) for 24 h. (c) Pairwise correlation between ROS generation and cell viability, proliferation, cell apoptosis, or DNA damage after treatment with vehicle and ALX (1.5, 3, and 6 mM) for 24 h, respectively. The correlations were analyzed by using Pearson analysis. NAC, a well-known ROS inhibitor, was used as a positive control. The data represent the mean ± SD (n = 3). ^∗∗∗p < 0.001 compared with the control group.