Figure 6.

ALX significantly inhibited the Keap1/Nrf2 signaling pathway in pancreatic β-cells. (a) The protein levels of Keap1, Nrf2, HO-1, and NQO1 in whole-cell lysates after treatment with vehicle and ALX (1.5, 3, and 6 mM) for 24 h were detected by Western blot analysis. (b) The nuclear Nrf2 protein levels were also detected after the same treatment. (c) Representative confocal images (scale bar: 50 μm) of MIN6 cells subjected to the same treatments and stained for Nrf2 (red) and with DAPI (blue). The data represent the mean ± SD (n = 3). ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001 compared with the control group.