Skip to main content
. 2021 Feb 5;2021:7866720. doi: 10.1155/2021/7866720

Figure 7.

Figure 7

The Keap1/Nrf2 signaling pathway mediates ALX-induced pancreatic β-cell injury. The MIN6 cells were treated with vehicle and ALX (3 mM) in the presence or absence of Nrf2-specific siRNAs (siNrf2-1 or siNrf2-2); the control cells were only transfected with control siRNA (siCon). (a) Cell viability was determined by using an MTT assay. (b) Proliferation of MIN6 cells was verified by EdU assay. (c) The MIN6 cell apoptosis was detected by Annexin V-FITC/propidium iodide (PI) staining. (d) Representative comet tail images showing the DNA damage. (e) Representative confocal images (scale bar: 50 μm) of MIN6 cells stained for p-H2A.X (red) and with DAPI (blue). (f) Intracellular ROS levels were detected by flow cytometry. (g) Intracellular ROS generation was detected by fluorescence microscopy (scale bar: 50 μm). The data represent the mean ± SD (n = 3). ##p < 0.01 and ###p < 0.001 compared with the siCon group. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with the siCon plus ALX group.