Table 2.
The PCR cycling conditions for detection of E. coli and L. monocytogenes isolates based on uidA and hly genes, respectively and to detect both pathogens based on 16S-rRNA genes in this study
| The PCR cycling conditions for detection of E. coli and L. monocytogenes isolates based on uidA and hly genes | ||||||
|---|---|---|---|---|---|---|
| Steps | Temperature (ºC) | Time (Sec) | Cycles | |||
| E. coli | L. monocytogenes | E. coli | L. monocytogenes | E. coli | L. monocytogenes | |
| Denaturation | 95 | 94 | 30 | 60 | 34 | 35 |
| Annealing | 65 | 60 | 30 | 120 | ||
| Extension | 72 | 72 | 30 | 60 | ||
| Final extension | 72 | 72 | 300 | 420 | 1 | 1 |
| The PCR cycling conditions based on 16S-rRNA genes in this study to detect both pathogens | |||
|---|---|---|---|
| Stage | Temperature (ºC) | Time (s) Cycle | Cycle |
| Initial denaturation | 95 | 900 | 1 |
| Denaturation | 95 | 30 | 35 |
| Annealing | 66 | 30 | |
| Extension | 72 | 60 | |
| Final extension | 72 | 420 | 1 |