Table 2.
Primer sequences for screening of pathotypes in PCR
| Pathotype | Gene | Sequence (5′–3′) | PCR condition | Product size (bp) | Reference |
|---|---|---|---|---|---|
| STEC/EPEC/EHEC | stx1 | AGAGCGATGTTACGGTTTG TTGCCCCCAGAGTGGATG |
30 cycles: 94°C (30 s), 50°C (30 s), 72°C (30 s) | 388 | (18) |
| stx2 | TGGGTTTTTCTTCGGTATC GACATTCTGGTTGACTCTCTT |
30 cycles: 94°C (30 s), 50°C (30 s), 72°C (30 s) | 807 | ||
| eae | AGGCTTCGTCACAGTTG CCATCGTCACCAGAGGA |
30 cycles: 94°C (30 s), 50°C (30 s), 72°C (30 s) | 507 | ||
| ETEC | st1 | ATTTTTMTTTCTGTATTRTCTT CACCCGGTACARGCAGGATT |
40 cycles: 95°C (45 s), 50°C (1 min), 72°C (1 min) | 190 | (19) |
| lt1 | GGCGACAGATTATACCGTGC CGGTCTCTATATTCCCTGTT |
40 cycles: 95ºC (45 s), 50°C (1 min), 72°C (1 min) | 450 | ||
| EIEC | ipaH | GTTCCTTGACCGCCTTTCCGATACCGTC GCCGGTCAGCCACCCTCTGAGAGTAC |
40 cycles: 95°C (45 s), 50°C (1 min), 72°C (1 min) | 600 | (19) |
| NTEC | cnf1 | GGGGGAAGTACAGAAGAATTA TTGCCGTCCACTCTCACCAGT |
30 cycles: 94°C (1 min), 54°C (30 s), 72°C (1 min) | 1111 | (20) |
| cnf2 | TATCATACGGCAGGAGGAAGCACC GTCACAATAGACAATAATTTTCCG |
30 cycles: 94°C (1 min), 55°C (1 min), 72°C (1 min) | 1240 |