Figure 4.

APE1 negatively regulates levels of active Rac1. (A) Triplicate cultures of sham (ctr) or shRNA transduced T84 cells were left uninfected (UI) or infected with S. Typhimurium. Cell lysates analyzed for levels of active GTP-bound Rac1 by a pull down assay or total Rac1. APE1-deficient cells show significantly increased levels of active Rac1 relative to total levels of Rac1 as determined by the ratios of the densitometry from four independent experiments. (B) As observed with S. Typhimurium, AIEC-infected APE1 deficient T84 cells show significantly higher levels of active Rac1. All error bars are represented as SEM. *p < 0.05; **p < 0.01.