FIGURE 4.
Induction of the methylcitrate cycle-related gene and enzyme activity by RegX3 in M. smegmatis growing on propionate under the low-phosphate condition. (A) Transcriptional profiles of regX3 and the methylcitrate cycle-related genes in WT, i regX3, and OE regX3 strains of M. smegmatis grown in MOPS-propionate (10 mM) medium supplemented with 100 μM K2HPO4. Cells were harvested for RNA extraction at 6 and 12 h after incubation. 16S rRNA was selected as a reference and fold change was analyzed by qRT-PCR. (B) Total activity of MCS enzyme in diverse M. smegmatis strains grown on propionate. Strains were incubated and collected under the same conditions as tested for the qRT-PCR analysis. 10 μg total protein were used. Data are presented as means ± SEM calculated from three independent experiments. Unpaired two-tailed Student’s t-test, *p < 0.05; **p < 0.01; and ***p < 0.001.