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. 2021 Feb 2;12:619387. doi: 10.3389/fmicb.2021.619387

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Copyright © 2021 Pei, Qi, Li, Wo and Ye.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Survival of diverse M. smegmatis strains in macrophages. THP-1-derived macrophage cells were infected with M. smegmatis WT, i regX3, and OE regX3 strains. Survival of these strains were determined in different time, 2, 12, 24, 36, and 48 h. Cells were harvested at different time points to determine the concentrations of live M. smegmatis (n = 6). Data are presented as means ± SEM calculated from three independent experiments. Unpaired two-tailed Student’s t-test, **p < 0.01 and ***p < 0.001.