TABLE 1.
Strains and plasmids used in this study.
| Strains/plasmids | Relevant characteristics | References |
| Strains | ||
| Mycobacterium smegmatis MC2 155 | Wild-type strain | Jenkins et al., 2012 |
| Mycobacterium smegmatis i regX3 | regX3 transcription inhibition strain; gentamycin resistant | This study |
| Mycobacterium smegmatis OE regX3 | regX3 overexpression strain carrying pMV261-regX3 | This study |
| Escherichia coli | ||
| DH5α | Recipient for cloning experiments | Novagen |
| BL21(DE3) | F– ompT hsdS gal dcm (DE3) | Novagen |
| Plasmids | ||
| pET28a(+) | Expression vector; Kanr | Novagen |
| pET28a-regX3 | pET28a derivative carrying regX3 of M. smegmatis | This study |
| pET28a-prpR | pET28a derivative carrying prpR of M. smegmatis | This study |
| pPR27 | Escherichia coli- M. smegmatis integrative shuttle vector; gentamycin resistant | Yao et al., 2016 |
| pPR27-hsp60-dCas9-sgRNA | regX3 transcription inhibition plasmid; gentamycin resistant | This study |
| pMV261 | Escherichia coli- M. smegmatis integrative shuttle vector containing a strong constitutive hsp60 promoter; kanamycin resistant | Yao and Ye, 2016 |
| pMV261-regX3 | pMV261 carrying an extra regX3 for gene overexpression | This study |