Fig. 4.

UBE2S and TRIM28 enhance the ubiquitination of p27 and regulate cell cycle progression. a Expression of p27 assessed by western blot analysis in HCC cells with UBE2S or TRIM28 silencing. b Endogenous interaction of UBE2S, TRIM28 and p27 in HCC cells. c–d Ubiquitination of p27 in HEK293T cells with UBE2S and TRIM28 overexpression or knockdown in the presence of MG132 (10 μM, 12 h) detected by immunoprecipitation and western blot analysis. e Differential expressions of cell cycle markers, including CDK2, CDK4, cyclin D1, and cyclin E1, were evaluated in HCC cells in which UBE2S and TRIM28 were elevated or silenced