Skip to main content
. 2021 Feb 15;11:3847. doi: 10.1038/s41598-021-83222-z

Table 1.

List of antibodies for western blot or immunocytochemistry.

Antibody Host Target MW (kDa) Dilution factor Application Manufacturer
#Cat. ID
Anti-JAK1 Rabbit polyclonal  ~ 130 1:500 WB1

Cell Signaling

#3332

Anti-JAK2 Rabbit polyclonal  ~ 125

1:500

1:5000

WB1

WB2

Merck Millipore

#06–255

Anti-Phospho-JAK1 (Tyr1034/1035) Rabbit polyclonal  ~ 130 1:1000 WB1

Cell Signaling

#3331

Anti-Phospho-JAK2 (Tyr1007/1008) Rabbit polyclonal  ~ 130

1:1000

1:5000

WB1

WB2

Merck Millipore

#09–275

Anti-Gfap Mouse monoclonal  ~ 50

1:1000

1:500

WB1

WB2; ICC

Merck Millipore

#MAB360

Anti-Tuj1 Rabbit polyclonal  ~ 55

1:1000

1:500

WB1

WB2; ICC

Sigma-Aldrich

#T2200

Anti-β-actin Rabbit polyclonal  ~ 45 1:1000 WB2

Cell Signaling

#4967

Anti-β-actin Goat polyclonal  ~ 45 1:1000 WB1

Santa Cruz

#sc-1616

Anti-Gapdh Mouse monoclonal  ~ 36 1:1000 WB1

Abcam

#ab125247

Anti-rabbit IgG, HRP-linked Goat - 1:2500 WB

Cell Signaling

#7074

Anti-mouse IgG, HRP-linked Goat - 1:3000 WB

Santa Cruz

#sc-2005

Anti-goat IgG, HRP-linked Donkey - 1:3000 WB

Santa Cruz

#sc-2020

Alexa Fluor 488 donkey anti-rabbit IgG Donkey - 1:1000 ICC

Life technologies

#A21206

Alexa Fluor 555 donkey anti-mouse Donkey - 1:1000 ICC

Life Technologies

#A31570

WB1 refers to immunoblotting for brain tissue lysates from P1.5 pups (Fig. 2); WB2 refers to immunoblotting for protein lysates from differentiating neurospheres (Fig. 3); ICC refers to immunocytochemistry.