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. 2021 Feb 15;12:1036. doi: 10.1038/s41467-021-21225-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a The genetic markers delimiting the Rf1 interval on chromosome 1A (chr1A) were genotyped and mapped in three populations segregating for male-fertility restoration conferred by the Rf1 gene. The 0.8 Mbp region delimited by marker cfn0522096 and cfn0527067 is indicated and the RFL genes present in the interval are shown. b The genetic markers delimiting the Rf3 interval on chromosome 1B (chr1B) were genotyped and mapped in three populations segregating for Rf3 and a doubled-haploid line carrying the Rf3 gene. The 2.8 Mbp region delimited by marker cfn1249269 and BS00090770 is indicated and the RFL genes present in the interval are shown. Scaffold sequences were obtained from the URGI IWGSC sequence repository (https://wheat-urgi.versailles.inra.fr/). Gene sequences that are truncated or disrupted by a frameshift are indicated by lighter colouring. The proposed order of the genes in the Rf3 interval in the Rf3 and Fielder line is only approximate. cM centimorgan, Mbp megabase pair. The marker sequences are listed in Supplementary Table 1.