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. 2021 Feb 15;12:1036. doi: 10.1038/s41467-021-21225-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Constructs used in the experiment. b Images showing complete male sterility induced by expression of the ZmMac2::Barnase construct in wheat. Red arrow shows aborted anther with only filament. c Plant fertility is restored by expression of ZmMac2::RFL29a. Plot marker area is proportional to the total number of transformation events obtained, ranging from 19 to 20. d Pollen from ZmMac2::RFL29a plants stained for viability, showing substantial restoration of normal pollen development. This experiment was performed once. A representative from 20 micrographs is shown. Scale bar = 200 μm. e Pollen viability counts. Plot marker areas are proportional to the number of grains counted, ranging from 920 to 3142. Source data underlying Fig. 4b–e are provided as a Source data file.