Table 2.
Purification of enterocin OS13.
| Purification step | Vol (mL) | Activity per mL (AU/mL)a | Total activity (AU) | Recovery (%) |
|---|---|---|---|---|
| Culture supernatant | 2000 | 64,000 | 128,000,000 | 100 |
| Ammonium sulfate precipitate | 40 | 4,096,000 | 163,840,000 | 128 |
| Ion exchange chromatography | 20 | 2,560,000 | 51,200,000 | 40 |
| First reversed-phase chromatography (RPC1 column) | 2 | 12,288,000 | 24,576,000 | 19.2 |
| Second reversed-phase chromatography (sephasil peptide C8 column) | ||||
| Enterocin OS13α | 1 | 6,144,000 | 6,144,000 | 4.8 |
| Enterocin OS13β | 1 | 4,096,000 | 4,096,000 | 3.2 |
a(AU/mL), Arbitrary unit per mL.