Table 1.
Guide RNAs.
| gRNA | Sequence | Target |
|---|---|---|
| TKO1 gRNA FW | CACCGTGCTAGAATAGCCTCCGATG | iso3 & iso4 |
| TKO1 gRNA RV | AAACCATCGGAGGCTATTCTAGCAC | |
| TKO2 gRNA FW | CACCGGGAACGAAAACTTCGCTTCA | iso3 & iso4 |
| TKO2 gRNA RV | AAACTGAAGCGAAGTTTTCGTTCCC | |
| 3KO1 gRNA FW | CACCGCAGTAGTGGTTCCATCAGGT | iso3 |
| 3KO1 gRNA RV | AAACACCTGATGGAACCACTACTC | |
| 3KO2 gRNA FW | CACCGCAAAACACAGCCGTGGCGGA | iso3 |
| 3KO2 gRNA RV | AAACTCCGCCACGGCTGTGTTTTGC | |
| 4KO1 gRNA FW | CACCGTGCGCGGCGATGAACTGCTT | iso4 |
| 4KO1 gRNA RV | AAACAAGCAGTTCATCGCCGCGCAC | |
| 4KO2 gRNA FW | CACCGCGGCGTGCCGTGGCCCGACG | iso4 |
| 4KO2 gRNA RV | AAACCGTCGGGCCACGGCACGCCGC |