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. 2021 Feb 2;9:635728. doi: 10.3389/fcell.2021.635728

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Copyright © 2021 Qin, Qu, Yuan, Li and Qiao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of RBM14 MO on the maturation of mouse oocytes. (A) Western blot analysis verified the efficiency of endogenous RBM14 knockdown. (B) RBM14 band intensities normalized to α-tubulin. (C) Representative bright-field images of RBM14-MO injected and control oocytes. Black rhombuses: successful polar body extrusion in control oocytes; black arrow: oocyte with apparent symmetric division; black asterisk: oocyte failed to extrude a polar body. Scale bar = 200 μm. (D) Rate of GVBD in control and RBM14-MO injected oocytes. (E) Rate of polar body extrusion in control and RBM14-MO injected oocytes. (F) Rate of symmetric division in control and RBM14-MO injected oocytes. A total of 364 control oocytes and 332 RBM14-MO injected oocytes were analyzed. Data are mean ± SEM of three independent experiments. NS, not significant, *P < 0.05 and **P < 0.01.