Figure 2.

CD8⁺ Trm cells in the CNS undergo local homeostatic proliferation. Two groups of mice were inoculated i.c. with AdIi-GP and 10 days prior to brain extraction half of the mice were administered FTY720 in their drinking water. Eight days before brain extraction, all mice were administered EdU in their drinking water or in the FTY720/drinking water mixture, respectively. (A) The number of CNS infiltrating CD8⁺ T cells and antigen specific (tetramer⁺) CD8⁺ T cells were measured for both groups. Each dot represents an individual. Bars represent median. P>0.05 illustrated by * (B) The percentage of EdU positive CD8⁺ and antigen specific CD8⁺ T cells were analyzed day 80 post i.c. to compare EdU incorporation in the presence of FTY720 or when recruitments were allowed. Percentages and representative plots of 4–5/group are shown. Results are representative of 2 experiments.