Figure 4.

Recruited immune and local CNS cells express PD-L1 during acute CNS inflammation, but expression declines over time. Following inoculation with AdIi-GP i.c, infiltrating neutrophils, monocytes and macrophages as well microglial cells were categorized based on the expression of CD45.2 and CD11b. The CD45^lowCD11b^low cells were further subdivided into 04⁺ oligodendrocytes and ASCA2⁺ astrocytes. (A) Representative plots of 4-5 mice at each time point, showing the gating of the three major cell-subsets for naïve mice and mice taken at two time-points post inoculation. For representative plot showing the subdivision into olidendrocytes and astrocytes, please see Supplementary Figure 2 . (B) Representative histogram of PD-L1 expression by the above mentioned cell subsets at day 0 (naïve mice), 11, 60 and 105, respectively. FMOs are shown in all histograms to indicate background. Medians of mean fluorescence intensities and ranges of PD-L1 expression for groups of 4-5 mice are included.