Figure 6.

PD-1 dampens the T-cell mediated immunopathology caused by i.c. inoculation of AdIi-GP. Three groups of mice were inoculated i.c. with AdIi-GP. Two of the groups were administered FTY720 in their drinking water. One of the groups was additionally depleted from CD8⁺ T cells as described in M&M. (A) The percentage of initial bodyweight lost during the period from zero - 11 days post i.c. is depicted for all groups. (B) C57BL6 mice were inoculated i.c. with AdIi-GP and half of the mice were administered FTY720 prior to inoculation. The number of CNS infiltrating CD8⁺ and antigen specific CD8⁺ T cells extracted day 7 post i.c. is shown. Each dot represents an individual. Bars represent median. (C) C57BL/6 – and PD-1 KO mice were inoculated i.c. with AdIi-GP. Additionally, a control group of C57BL/6 mice were i.c inoculated with PBS. Weights were monitored from day zero - 11 and the percentage of initial bodyweight lost is shown. Means and standard deviations for 4-30 animals per group pooled from 3 experiments. For the weight curves, AUC was calculated for all mice and summed for each group. A significant difference in the AUC between the groups is indicated by * if P<0.05.