Figure 9.

Ex vivo functional capacity of the PD-1 positive CD8⁺ T cell in the memory stage. C57BL/6 mice were inoculated with AdIi-GP i.c and at day 40 post i.c. numbers of CNS derived CD8⁺ T cells producing IFN-γ, IFN-γ and TNF-α – or IL-2 in response to in vitro GP_33-41 or GP_276-281 stimulation were enumerated as shown. (A) Unstimulated samples were used to set the background. (B) The mean fluorescence intensity (MFI) of the IFN-γ response was calculated for CD8⁺ T cells extracted from both brain and spleen. Unstimulated samples were included as control. (C) representative plots of cytokine producing CD8⁺ T cells in presence or absence of peptide stimulation. Results are representative of 2–3 experiments involving 4-5 mice/group.