Figure 3.

Targeting purinergic receptor P2RX1 alleviated inflammatory responses in acute pancreatitis. (A) Caerulein (50 μg/kg) was intraperitoneally injected seven times hourly to induce acute pancreatitis in WT and P2RX1-KO mice. NF449 or vehicle was intraperitoneally injected immediately after the first injection of caerulein. Saline-treated mice were set as negative control (NC). Pancreas tissues from each group were harvested at 8 h, stained with H&E, and observed under a microscope (100×). Representative images were shown. Scale bar is 50 μm. (B) At 8 h after caerulein exposure, serum amylase activity, pancreas IL1-β mRNA expression, Cxcl1 mRNA expression, myeloperoxidase (MPO) activity, and malondialdehyde (MDA) were analyzed to evaluate inflammatory status (n = 5 per group). 2^{− δδ Ct} value was used for comparisons of the fold change of mRNA expression to NC mice. Statistics were calculated using one-way ANOVA with Tukey post-tests. *P < 0.05, **P < 0.01, ***P < 0.001.